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| Captain Dominic DeLuca - Commanding Officer Species: Human-Klingon / Portrayed by: David Boreanaz |
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| Lieutenant Commander Jaarid - Executive Officer Species: R'naari / Portrayed by: Jared Padalecki |
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| Commander Maureen Killian, M.D. - Chief Medical Officer Species: Human / Portrayed by: Gillian Anderson |
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| Lieutenant Commander Arkhet djan Zabrak - Chief Engineer Species: Tellarite / Portrayed by: Kevin James |
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| Lieutenant Tyrone Ja-Nareth - Senior Science Officer Species: Efrosian / Portrayed by: T.J. Thyne |
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| Lieutenant Sullek - Senior Operations Officer Species: Roylan |
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| Lieutenant Yvala Hollen - Chief of Security/Tactical Officer Species: Trill (Unjoined) / Portrayed by: Holly Valance |
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| Ensign Charlaine Tucker - Senior Flight Control Officer Species: Human / Portrayed by: Anna Kendrick |
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| Dr. Amalys Tyr'lylth, Ph.D. - Ship's Counselor Species: Muus-a / Portrayed by: Bridget Regan |
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